BrdU,5-溴脱氧尿嘧啶核苷抗体

产品编号HZ-0489R
英文名称BrdU
中文名称5-溴脱氧尿嘧啶核苷抗体
别 名Bromodeoxyuridine; Bromodeoxyuridine; 5-Bromo-2′-deoxyuridine; 5-BrdU; Proliferation Marker; 5-Bromo-2-deoxyUridine.
说 明 书0.1ml 0.2ml
产品类型药物与化合物抗体
研究领域细胞生物
抗体来源Rabbit
克隆类型Polyclonal
交叉反应BrdU
BrdU,5-溴脱氧尿嘧啶核苷抗体产品应用ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
细胞定位细胞核
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原BrdU conjugated to KLH
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
BrdU,5-溴脱氧尿嘧啶核苷抗体PubMedPubMed
产品介绍background:
The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis.
BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis

Subcellular Location:
Nuclear.

CAS:
59-14-3

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

BrdU,5-溴脱氧尿嘧啶核苷抗体Proliferation Marker
BrdU（溴化脱氧尿嘧啶核苷）可以在体内和体外掺入到处于S期的细胞所合成的DNA链中。 此抗体可以与掺入到任何种属细胞中的BrdU反应，与碘脱氧尿 嘧啶有交叉反应，标记掺有BrdU的S期细胞，主要用于研究各种 不同的因素对正常/肿瘤组织的细胞增殖及动力学的研究。
用FITC标记的抗BrdU IgG可用于流式细胞术定量检测增值细胞。