RING1 polyclonal antibody
产品名称： RING1 polyclonal antibody
英文名称： RING1 polyclonal antibody
产品编号： PAB4359
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of RING1.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human RING1.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- RING1 is associated with the polycomb group protein complex and acts as a transcriptional repressor.
Satijn DP, Gunster MJ, van der Vlag J, Hamer KM, Schul W, Alkema MJ, Saurin AJ, Freemont PS, van Driel R, Otte AP.Mol Cell Biol. 1997 Jul;17(7):4105-13.
- 2.
- Physical mapping 220 kb centromeric of the human MHC and DNA sequence analysis of the 43-kb segment including the RING1, HKE6, and HKE4 genes.
Kikuti YY, Tamiya G, Ando A, Chen L, Kimura M, Ferreira E, Tsuji K, Trowsdale J, Inoko H.Genomics. 1997 Jun 15;42(3):422-35.
- 3.
- Identification and preliminary characterization of a protein motif related to the zinc finger.
Lovering R, Hanson IM, Borden KL, Martin S, O'Reilly NJ, Evan GI, Rahman D, Pappin DJ, Trowsdale J, Freemont PS.Proc Natl Acad Sci U S A. 1993 Mar 15;90(6):2112-6.
- Applications
- Western Blot (Cell lysate)
- The RING1 polyclonal antibody (Cat # PAB4359) is used in Western blot to detect RING1 in Jurkat cell lysate .
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with RING1 polyclonal antibody (Cat # PAB4359) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . HC = hepatocarcinoma .
- Entrez GeneID:
- 6015
- Protein Accession#:
- Q06587
- Gene Name:
- RING1
- Gene Alias:
- RING1A,RNF1
- Gene Description:
- ring finger protein 1
- Omim ID:
- 602045
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene belongs to the RING finger family, members of which encode proteins characterized by a RING domain, a zinc-binding motif related to the zinc finger domain. The gene product can bind DNA and can act as a transcriptional repressor. It is associated with the multimeric polycomb group protein complex. The gene product interacts with the polycomb group proteins BMI1, EDR1, and CBX4, and colocalizes with these proteins in large nuclear domains. It interacts with the CBX4 protein via its glycine-rich C-terminal domain. The gene maps to the HLA class II region, where it is contiguous with the RING finger genes FABGL and HKE4. [provided by RefSeq
- Other Designations:
- OTTHUMP00000029416