TWIST1 monoclonal antibody (M01), clone 3E11
产品名称： TWIST1 monoclonal antibody (M01), clone 3E11
英文名称： TWIST1 monoclonal antibody (M01), clone 3E11
产品编号： H00007291-M01
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Mouse monoclonal antibody raised against a partial recombinant TWIST1.
- Immunogen:
- TWIST1 (NP_000465, 100 a.a. ~ 202 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
- Sequence:
- PQSYEELQTQRVMANVRERQRTQSLNEAFAALRKIIPTLPSDKLSKIQTLKLAARYIDFLYQVLQSDELDSKMASCSYVAHERLSYAFSVWRMEGAWSMSASH
- Host:
- Mouse
- Reactivity:
- Human, Rat
- Isotype:
- IgG1 Kappa
- Storage Buffer:
- In 1x PBS, pH 7.2
- Storage Instruction:
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (37.07 KDa) .
- MSDS:
Download
- Publication Reference
- 1.
- EMT and induction of miR-21 mediate metastasis development in Trp53-deficient tumours.
Bornachea O, Santos M, Martinez-Cruz AB, Garcia-Escudero R, Duenas M, Costa C, Segrelles C, Lorz C, Buitrago A, Saiz-Ladera C, Agirre X, Grande T, Paradela B, Maraver A, Ariza JM, Prosper F, Serrano M, Sanchez-Cespedes M, Paramio JM.Sci Rep. 2012;2:434. Epub 2012 May 31.
- 2.
- Conversion of Helicobacter pylori CagA from senescence inducer to oncogenic driver through polarity-dependent regulation of p21.
Saito Y, Murata-Kamiya N, Hirayama T, Ohba Y, Hatakeyama M.J Exp Med. 2010 Sep 27;207(10):2157-74. Epub 2010 Sep 20.
- Applications
- Western Blot (Tissue lysate)
- TWIST1 monoclonal antibody (M01), clone 3E11. Western Blot analysis of TWIST1 expression in rat brain.
- Protocol Download
- Western Blot (Transfected lysate)
- Western Blot analysis of TWIST1 expression in transfected 293T cell line by TWIST1 monoclonal antibody (M01), clone 3E11.
Lane 1: TWIST1 transfected lysate(21 KDa).
Lane 2: Non-transfected lysate. - Protocol Download
- Western Blot (Recombinant protein)
- Protocol Download
- Immunoprecipitation
- Immunoprecipitation of TWIST1 transfected lysate using anti-TWIST1 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with TWIST1 MaxPab rabbit polyclonal antibody.
- Protocol Download
- Sandwich ELISA (Recombinant protein)
- Detection limit for recombinant GST tagged TWIST1 is approximately 3ng/ml as a capture antibody.
- Protocol Download
- RNAi Knockdown (Antibody validated)
- Western blot analysis of TWIST1 over-expressed 293 cell line, cotransfected with TWIST1 Validated Chimera RNAi ( Cat # H00007291-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TWIST1 monoclonal antibody (M01), clone 3E11 (Cat # H00007291-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Protocol Download
- Entrez GeneID:
- 7291
- GeneBank Accession#:
- NM_000474
- Protein Accession#:
- NP_000465
- Gene Name:
- TWIST1
- Gene Alias:
- ACS3,BPES2,BPES3,SCS,TWIST,bHLHa38
- Gene Description:
- twist homolog 1 (Drosophila)
- Gene Ontology:
- Hyperlink
- Gene Summary:
- Basic helix-loop-helix (bHLH) transcription factors have been implicated in cell lineage determination and differentiation. The protein encoded by this gene is a bHLH transcription factor and shares similarity with another bHLH transcription factor, Dermo1. The strongest expression of this mRNA is in placental tissue; in adults, mesodermally derived tissues express this mRNA preferentially. Mutations in this gene have been found in patients with Saethre-Chotzen syndrome. [provided by RefSeq
- Other Designations:
- B-HLH DNA binding protein,H-twist,TWIST homolog of drosophila,twist