预优化DNA转染试剂-U2OS
产品名称： 预优化DNA转染试剂-U2OS
英文名称： GenJet™ In Vitro DNA Tranfection Reagent for U2OS Cells
产品编号： SL100489-OS
产品价格： 0
产品产地： 美国
品牌商标： SignaGen
更新时间： null
使用范围： null
济南思科生物科技有限公司
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Description
GenJet™ DNA In Vitro Tranfection Reagent for U2OS cells is pre-optimized for transfecting U2OS cells. The U2OS cell line, originally known as the 2T line, was cultivated from the bone tissue of a fifteen-year-old human female suffering from osteosarcoma. Established in 1964, the original cells were taken from a moderately differentiated sarcoma of the tibia. U2 OS cells exhibit epithelial adherent morphology and viruses were not detected in the line during co-cultivation with WI-38 cells or in CF tests against SV40, RSV, or adenoviruses. Cells are positive for insulin-like growth factor I (IGF-I) and insulin-like growth factor II (IGF II) receptors and express a number of antigens, including blood type A, Rh+, HLA A2, Aw30, B12, Bw35, and B40(+/-).
Refer to the following optimal transfection conditions for maximal transfection efficiency on U2 OS cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 150 to 300 transfections in 6 well plates.
Refer to the following optimal transfection conditions for maximal transfection efficiency on U2 OS cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 150 to 300 transfections in 6 well plates.
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Summary of Optimal Transfection Conditions:
Confluence on the day of transfection Cell culture conditions GenJet™ (µl) : DNA (µg) Ratio Diluent for DNA and Transfection Reagent Incubation Time to Form GenJet™/DNA Complex Presence of Serum/Antibiotics during Transfection Change Medium 5 Hours After Transfection Maximal Efficiency Transfection Results: Reporter Gene Plasmid Efficiency (GFP %) |
60~70% DMEM with 4.5 g/L glucose, 10% FBS 3:1 Serum-free DMEM with 4.5 g/L glucose 15 minutes at RT Yes No 48 hours EGFP pEGFP-N3 (CMV promoter) 95% |
Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer
A Picture Showing Transfection Efficiency of GenJet™ Reagent on U2 OS Cells
Store at 4 °C. If stored properly, the product is stable for 12 months or longer
A Picture Showing Transfection Efficiency of GenJet™ Reagent on U2 OS Cells
GenJet™ reagent is optimized for U2OS. U2OS cells were grown per ATCC recommended culture medium and transfected with pEGFP-N3 by GenJet™. The efficiency was checked 48 hours post transfection
Data Sheet.pdf