HDAC9 polyclonal antibody
产品名称： HDAC9 polyclonal antibody
英文名称： HDAC9 polyclonal antibody
产品编号： PAB2339
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of HDAC9.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human HDAC9.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
Immunoprecipitation (1:50-100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Molecular characterization of a familial translocation implicates disruption of HDAC9 and possible position effect on TGFbeta2 in the pathogenesis of Peters' anomaly.
David D, Cardoso J, Marques B, Marques R, Silva ED, Santos H, Boavida MG.Genomics. 2003 May;81(5):489-503.
- 2.
- The histone deacetylase 9 gene encodes multiple protein isoforms.
Petrie K, Guidez F, Howell L, Healy L, Waxman S, Greaves M, Zelent A.J Biol Chem. 2003 May 2;278(18):16059-72. Epub 2003 Feb 17.
- 3.
- Chromosomal organization and localization of the human histone deacetylase 9 gene (HDAC9).
Mahlknecht U, Schnittger S, Will J, Cicek N, Hoelzer D.Biochem Biophys Res Commun. 2002 Apr 26;293(1):182-91.
- Applications
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with the HDAC9 polyclonal antibody (Cat # PAB2339) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
- Immunoprecipitation
- Both anti-HDAC9 N-term (Cat # PAB2238) and C-term (Cat # PAB2339) polyclonal antibody were tested by WB and IP-WB using HeLa and HeLa-HDAC9 transfected cells.
Top figure shows both polyclonal antibody specifically detect HDAC9 in HeLa-HDAC9 transfected cell but not HeLa alone.
Bottom figure shows that both polyclonal antibody can immunoprecipitate (IP) HDAC9 from HeLa-HDAC9 tranfected cells.
(Data kindly provided by Dr. Zhigang Yuan, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL) .
- Entrez GeneID:
- 9734
- Protein Accession#:
- Q9UKV0
- Gene Name:
- HDAC9
- Gene Alias:
- DKFZp779K1053,HD7,HDAC,HDAC7,HDAC7B,HDAC9B,HDAC9FL,HDRP,KIAA0744,MITR
- Gene Description:
- histone deacetylase 9
- Omim ID:
- 606543
- Gene Ontology:
- Hyperlink
- Gene Summary:
- Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene has sequence homology to members of the histone deacetylase family. This gene is orthologous to the Xenopus and mouse MITR genes. The MITR protein lacks the histone deacetylase catalytic domain. It represses MEF2 activity through recruitment of multicomponent corepressor complexes that include CtBP and HDACs. This encoded protein may play a role in hematopoiesis. Multiple alternatively spliced transcripts have been described for this gene but the full-length nature of some of them has not been determined. [provided by RefSeq
- Other Designations:
- MEF-2 interacting transcription repressor (MITR) protein,histone deacetylase 4/5-related protein,histone deacetylase 7,histone deacetylase 7B