CDC25A (phospho S75) polyclonal antibody
产品名称： CDC25A (phospho S75) polyclonal antibody
英文名称： CDC25A (phospho S75) polyclonal antibody
产品编号： PAB0429
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of CDC25A.
- Immunogen:
- Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S75 of human CDC25A.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Cdc25A and cdc25B expression in malignant lymphoma of the thyroid: correlation with histological subtypes and cell proliferation.
Ito Y, Yoshida H, Matsuzuka F, Matsuura N, Nakamura Y, Nakamine H, Kakudo K, Kuma K, Miyauchi A.Int J Mol Med. 2004 Mar;13(3):431-5.
- 2.
- Activation of the Raf-1/MEK/Erk kinase pathway by a novel Cdc25 inhibitor in human prostate cancer cells.
Nemoto K, Vogt A, Oguri T, Lazo JS.Prostate. 2004 Jan 1;58(1):95-102.
- 3.
- Regulation of human Cdc25A stability by Serine 75 phosphorylation is not sufficient to activate a S phase checkpoint.
Goloudina A, Yamaguchi H, Chervyakova DB, Appella E, Fornace AJ Jr, Bulavin DV.Cell Cycle. 2003 Sep-Oct;2(5):473-8.
- Applications
- Western Blot (Tissue lysate)
- The CDC25A (phospho S75) polyclonal antibody (Cat # PAB0429) is used in Western blot to detect Phospho-CDC25A-S75 in mouse kidney tissue lysate.
- Western Blot (Cell lysate)
- The CDC25A (phospho S75) polyclonal antibody (Cat # PAB0429) is used in Western blot to detect Phospho-CDC25A-S75 in Hela (left) and HL-60 (right) cell lysates.
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with CDC25A (phospho S75) polyclonal antibody (Cat # PAB0429) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
- Entrez GeneID:
- 993
- Protein Accession#:
- NP_001780;P30304
- Gene Name:
- CDC25A
- Gene Alias:
- CDC25A2
- Gene Description:
- cell division cycle 25 homolog A (S. pombe)
- Omim ID:
- 116947
- Gene Ontology:
- Hyperlink
- Gene Summary:
- CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
- Other Designations:
- M-phase inducer phosphatase 1,cell division cycle 25A,dual specificity phosphatase CDC25A
- Interactome
- Gene Pathway