癌胚抗原单克隆抗体（检测）
产品名称： 癌胚抗原单克隆抗体（检测）
英文名称： CEA(B5)
产品编号： hzm-1624M
产品价格： null
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围： ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500
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Mouse Anti-CEA(B5) antibody
| 产品编号 | hzm-1624M |
| 英文名称 | CEA(B5) |
| 中文名称 | 癌胚抗原单克隆抗体（检测） |
| 别 名 | Carcinoembryonic antigen; Carcinoembryonic antigen related cell adhesion molecule 5; Carcinoembryonic antigen-related cell adhesion molecule 5; CD66e; CD66e; CD66e antigen; CEA; CEA; CEACAM5; CEAM5_HUMAN; DKFZp781M2392; Meconium antigen 100; OTTHUMP00000199032; OTTHUMP00000199033; OTTHUMP00000199034. |
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| 研究领域 | 肿瘤 细胞类型标志物 |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | B5 |
| 交叉反应 | Human, |
| 产品应用 | ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 150-200kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Human Carcino Embryonic Antigen:full length |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M PBS(pH7.4) with 0.03% Proclin300 |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: CEA-related cell adhesion molecules (CEACAM) belong to the carcinoembryonic antigen (CEA) family. It consists of seven CEACAM (CEACAM 1, CEACAM 3-CEACAM 8) and 11 pregnancy-specific glyco-protein (PSG 1-PSG 11) members. The CEA family proteins belong to the immunoglobulin (Ig) superfamily and are composed of one Ig variable-like (IgV) and a varying number (0-6) of Ig constant-like (IgC) domains. CEACAM molecules are membrane-bound either via a transmembrane domain or a glycosyl phosphatidyl inositol (GPI) anchor. CEACAM molecules are differentially expressed in epithelial cells or in leucocytes. Over-expression of CEA/ CEACAM 5 in tumors of epithelial origin is the basis of its wide-spread use as a tumor marker. The function of CEACAM family members varies widely: they function as cell adhesion molecules, tumor suppressors, regulators of lymphocyte and dendritic cell activation, receptors of Neisseria species and other bacteria. Function: Cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. Receptor for E.coli Dr adhesins. Subunit: Homodimer. Binding of E.coli Dr adhesins leads to dissociation of the homodimer. Subcellular Location: Cell membrane; Lipid-anchor, GPI-anchor Tissue Specificity: Found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon. Post-translational modifications: Complex immunoreactive glycoprotein with a MW of 180 kDa comprising 60% carbohydrate. Similarity: Belongs to the immunoglobulin superfamily. CEA family. Contains 7 Ig-like (immunoglobulin-like) domains. SWISS: P06731 Gene ID: 1048 Database links: Entrez Gene: 1048 Human Omim: 114890 Human SwissProt: P06731 Human Unigene: 709196 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. CEA是一种胚胎性抗原，主要存在于胎儿消化道上皮组织，胰腺和肝癌中。CEA广泛存在各种上皮性肿瘤，尤其是胃肠道恶性肿瘤，CEA分布阳性类型与肿瘤的恶性度有关。CEA与EMA一样可作为上皮性恶性肿瘤的重要标记。 分子量为150-200 kDa。 该抗体与bsm-1623M（检测）（Mouse Anti-Human CEA Monoclonal）配对使用；主要用于ELISA试剂盒，胶体金试剂条制备生产都很好。大量订购可来电商议。 |
| 产品图片 |
 Paraformaldehyde-fixed, paraffin embedded (liver cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CEA(B5)) Polyclonal Antibody, Unconjugated (bsm-1624M) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0024) for 20 minutes and DAB staining.
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