DAAM1 monoclonal antibody (M05), clone 5D3
产品名称： DAAM1 monoclonal antibody (M05), clone 5D3
英文名称： DAAM1 monoclonal antibody (M05), clone 5D3
产品编号： H00023002-M05
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Mouse monoclonal antibody raised against a partial recombinant DAAM1.
- Immunogen:
- DAAM1 (NP_055807, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
- Sequence:
- MAPRKRGGRGISFIFCCFRNNDHPEITYRLRNDSNFALQTMEPALPMPPVEELDVMFSELVDELDLTDKHREAMFALPAEKKWQIYCSKKKDQEENKGATSWPEFYIDQL
- Host:
- Mouse
- Reactivity:
- Human, Rat
- Isotype:
- IgG2a Kappa
- Storage Buffer:
- In 1x PBS, pH 7.2
- Storage Instruction:
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (37.84 KDa) .
- MSDS:
Download
- Publication Reference
- 1.
- Molecular profile of endothelial invasion of three-dimensional collagen matrices: insights into angiogenic sprout induction in wound healing.
Su SC, Mendoza EA, Kwak HI, Bayless KJ.Am J Physiol Cell Physiol. 2008 Nov;295(5):C1215-29. Epub 2008 Sep 11.
- 2.
- Daam1 regulates the endocytosis of EphB during the convergent extension of the zebrafish notochord.
Kida YS, Sato T, Miyasaka KY, Suto A, Ogura T.Proc Natl Acad Sci U S A. 2007 Apr 17;104(16):6708-13. Epub 2007 Apr 5.
- Applications
- Western Blot (Cell lysate)
- DAAM1 monoclonal antibody (M05), clone 5D3. Western Blot analysis of DAAM1 expression in PC-12 ( Cat # L012V1 ).
- Protocol Download
- Western Blot (Cell lysate)
- DAAM1 monoclonal antibody (M05), clone 5D3 Western Blot analysis of DAAM1 expression in A-431 ( Cat # L015V1 ).
- Protocol Download
- Western Blot (Transfected lysate)
- Western Blot analysis of DAAM1 expression in transfected 293T cell line by DAAM1 monoclonal antibody (M05), clone 5D3.
Lane 1: DAAM1 transfected lysate(122 KDa).
Lane 2: Non-transfected lysate. - Protocol Download
- Western Blot (Recombinant protein)
- Protocol Download
- Sandwich ELISA (Recombinant protein)
- Detection limit for recombinant GST tagged DAAM1 is approximately 0.03ng/ml as a capture antibody.
- Protocol Download
- Entrez GeneID:
- 23002
- GeneBank Accession#:
- NM_014992
- Protein Accession#:
- NP_055807
- Gene Name:
- DAAM1
- Gene Alias:
- FLJ41657,KIAA0666
- Gene Description:
- dishevelled associated activator of morphogenesis 1
- Omim ID:
- 606626
- Gene Ontology:
- Hyperlink
- Gene Summary:
- Functions of the cell cortex, including motility, adhesion, and cytokinesis, are mediated by the reorganization of the actin cytoskeleton and recent evidence suggests a role for the Formin homology (FH) proteins in these processes. The protein encoded by this gene contains FH domains and belongs to a novel FH protein subfamily implicated in cell polarity. Wnt/Fz signaling activates the small GTPase Rho, a key regulator of cytoskeleton architecture, to control cell polarity and movement during development. Activation requires Dvl-Rho complex formation, an assembly mediated by this gene product, which is thought to function as a scaffolding protein. Evidence of alternative splicing has been observed for this gene but the full-length nature of these variants has not been determined. [provided by RefSeq
- Other Designations:
- OTTHUMP00000179033,dishevelled-associated activator of morphogenesis 1
- Gene Pathway
- Related Disease