The Human Stress & Toxicity PathwayFinder EpiTect Methyl II Signature PCR Array profiles the promoter methylation status of a panel of 22 genes indicative of cellular stress responses and toxicity. Cells respond to a variety of exogenous insults by activating stress pathways, and epigenetic mechanisms regulate some of these responses. Multiple studies have found gene-specific methylation changes in stress response pathways upon in vitro exposure to stress or toxic compounds. However, the mechanistic details and functional consequences have yet to be discovered or confirmed by researchers. Profiling cellular or fresh tissue genomic DNA samples with these arrays may help correlate CpG island methylation status with biological phenotypes, such as the ability of cells to mount specific stress responses. This gene panel focuses on epigenetically-regulated genes confirmed experimentally to show changes in promoter methylation status. The results may also help provide further insights into the molecular mechanisms behind cellular stress responses. With a simple restriction enzyme digestion and real-time PCR, research studies can analyze the promoter methylation status of 22 different genes central to stress and toxicity with this DNA methylation PCR array.

The EpiTect Methyl II PCR Arrays use the MethylScreen™ Technology provided under license from Orion Genomics, LLC.

Oxidative Stress:BNIP3, CYP1A1, GPX3, PRDX2, PTGS2 (COX2), SCARA3.

DNA Damage:ATM, BRCA1, CCND1, GADD45A, GADD45G, MLH1, MSH2, TP53, XPC.

Unfolded Protein Response:INSIG1, UBE2G2.

Hypoxia Signaling:CSTB, RARA.

Heat Shock Protein:DNAJC15.

Growth Arrest & Senescence:ATM, CDKN1A (p21CIP1/WAF1), GADD45A, GDF15 (PLAB), MSH2, TP53.

Inflammation:PRDX2, PTGS2 (COX2).

Anti-Apoptosis:BNIP3, CDKN1A (p21CIP1/WAF1), PRDX2.

Proliferation & Carcinogenesis:BRCA1, CCND1, CDKN1A (p21CIP1/WAF1), CYP1A1, PRDX2, PTGS2 (COX2), TP53