FITC标记的DNA损伤关卡蛋白1抗体
产品名称： FITC标记的DNA损伤关卡蛋白1抗体
英文名称： Anti-MDC1/FITC
产品编号： HZ-2400R-FITC
产品价格： null
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围： IF=1:50-200
上海沪震实业有限公司
- 联系人 : 鲍丽雯
- 地址 : 上海市闵行区闵北路88弄1-30号第22幢AQ136室
- 邮编 : 200612
- 所在区域 : 上海
- 电话 : 139****0749 点击查看
- 传真 : 点击查看
- 邮箱 : www.shzbio.net
- 二维码 : 点击查看
Rabbit Anti-MDC1/FITC Conjugated antibody
FITC标记的DNA损伤关卡蛋白1抗体
| 英文名称 | Anti-MDC1/FITC |
| 中文名称 | FITC标记的DNA损伤关卡蛋白1抗体 |
| 别 名 | NFBD1; Nuclear factor with BRCT domains protein 1; DKFZp781A0122; Homologue to Drosophila photoreceptor protein calphotin; KIAA0170; MGC166888; MDC 1; Mediation of DNA damage checkpoint 1; Mediator of DNA damage checkpoint 1; Mediator of DNA Damage Checkpoint Protein 1; NFBD 1; NFBD1; Nuclear Factor with BRCT Domains Protein 1; MDC1_HUMAN. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 细胞生物 神经生物学 信号转导 细胞凋亡 转录调节因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Dog, Pig, Cow, Horse, (MBS) |
| 产品应用 | IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 226(human), 188(mo), 147(rat)kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NFBD1 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: The protein encoded by this gene contains an N-terminal forkhead domain, two BRCA1 C-terminal (BRCT) motifs and a central domain with 13 repetitions of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs, and facilitates recruitment of the ATM kinase and meiotic recombination 11 protein complex to DNA damage foci. [provided by RefSeq]. Function: Required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. May serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked by 'Ser-139' phosphorylation of histone H2AFX. Also required for downstream events subsequent to the recruitment of these proteins. These include phosphorylation and activation of the ATM, CHEK1 and CHEK2 kinases, and stabilization of TP53 and apoptosis. ATM and CHEK2 may also be activated independently by a parallel pathway mediated by TP53BP1. Subunit: Interacts with several proteins involved in the DNA damage response, although not all these interactions may be direct. Interacts with H2AFX, which requires phosphorylation of H2AFX on 'Ser-139'. Interacts with the MRN complex, composed of MRE11A/MRE11, RAD50, and NBN. Interacts with CHEK2, which requires ATM-mediated phosphorylation of 'Thr-68' within the FHA domain of CHEK2. Interacts constitutively with the BRCA1-BARD1 complex, SMC1A and TP53BP1. Interacts with ATM and FANCD2, and these interactions are reduced upon DNA damage. Also interacts with the PRKDC complex, composed of XRCC6/KU70, XRCC5/KU80 and PRKDC/XRCC7. This interaction may be required for PRKDC autophosphorylation, which is essential for DNA double strand break (DSB) repair. When phosphorylated by ATM, interacts with RNF8 (via FHA domain). Interacts with CEP164. When phosphorylated, interacts with APTX (via FHA-like domain). Subcellular Location: Nucleus. Note=Associated with chromatin. Relocalizes to discrete nuclear foci following DNA damage, this requires 'Ser-139' phosphorylation of H2AFX. Colocalizes with APTX at sites of DNA double-strand breaks. Tissue Specificity: Highly expressed in testis. Post-translational modifications: Phosphorylated upon exposure to ionizing radiation (IR), ultraviolet radiation (UV), and hydroxyurea (HU). Phosphorylation in response to IR requires ATM, NBN, and possibly CHEK2. Also phosphorylated during the G2/M phase of the cell cycle and during activation of the mitotic spindle checkpoint. Similarity: Contains 2 BRCT domains. Contains 1 FHA domain. Database links: Entrez Gene: 9656 Human Entrez Gene: 240087 Mouse GenBank: NP_055456 Human Omim: 607593 Human SwissProt: Q14676 Human SwissProt: Q5PSV9 Mouse Unigene: 433653 Human Unigene: 653495 Human Unigene: 218511 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. NFBD1又称MDC1（mediator of DNA damagecheckpoint protein 1）是一个参与DNA损伤细胞应答通路的重要传递和修复因子,在DNA损伤关卡、DNA损伤修复、细胞生长增殖及肿瘤发生中均有作用 |
由该基因编码的蛋白质包含N-末端叉头结构域、两个BRCA1 C-末端（BRCT）基序和13个重复约41个氨基酸序列的中心结构域。编码的蛋白质需要激活内S期和G2/M期细胞周期检查点以响应DNA损伤。该核蛋白通过磷酸化的组蛋白H2AX通过其BRCT基序与DNA双链断裂位点相互作用，并促进ATM激酶和减数分裂重组11蛋白复合物向DNA损伤灶的募集。[由RefSeq提供]。