NFKB1 monoclonal antibody (M02), clone 4A11
产品名称： NFKB1 monoclonal antibody (M02), clone 4A11
英文名称： NFKB1 monoclonal antibody (M02), clone 4A11
产品编号： H00004790-M02
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Mouse monoclonal antibody raised against a partial recombinant NFKB1.
- Immunogen:
- NFKB1 (AAH51765, 860 a.a. ~ 969 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
- Sequence:
- MDNYEVSGGTVRELVEALRQMGYTEAIEVIQAASSPVKTTSQAHSLPLSPASTRQQIDELRDSDSVCDSGVETSFRKLSFTESLTSGASLLTLNKMPHDYGQEGPLEGKI
- Host:
- Mouse
- Reactivity:
- Human
- Isotype:
- IgG1 Kappa
- Storage Buffer:
- In 1x PBS, pH 7.2
- Storage Instruction:
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (37.73 KDa) .
- MSDS:
Download
- Applications
- Western Blot (Transfected lysate)
- Western Blot analysis of NFKB1 expression in transfected 293T cell line by NFKB1 monoclonal antibody (M02), clone 4A11.
Lane 1: NFKB1 transfected lysate(105.4 KDa).
Lane 2: Non-transfected lysate. - Protocol Download
- Western Blot (Recombinant protein)
- Protocol Download
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
enlarge this image- Immunoperoxidase of monoclonal antibody to NFKB1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 1.5 ug/ml]
- Protocol Download
- Immunofluorescence
enlarge this image- Immunofluorescence of monoclonal antibody to NFKB1 on HeLa cell . [antibody concentration 10 ug/ml]
- Protocol Download
- Sandwich ELISA (Recombinant protein)
- Detection limit for recombinant GST tagged NFKB1 is approximately 3ng/ml as a capture antibody.
- Protocol Download
- In situ Proximity Ligation Assay (Cell)
- Proximity Ligation Analysis of protein-protein interactions between HSP90AB1 and NFKB1 HeLa cells were stained with anti-HSP90AB1 rabbit purified polyclonal 1:1200 and anti-NFKB1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
- Entrez GeneID:
- 4790
- GeneBank Accession#:
- BC051765
- Protein Accession#:
- AAH51765
- Gene Name:
- NFKB1
- Gene Alias:
- DKFZp686C01211,EBP-1,KBF1,MGC54151,NF-kappa-B,NFKB-p105,NFKB-p50,p105,p50
- Gene Description:
- nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
- Omim ID:
- 164011
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
- Other Designations:
- DNA binding factor KBF1,NF-kappabeta,nuclear factor NF-kappa-B p50 subunit,nuclear factor kappa-B DNA binding subunit,nuclear factor kappa-B, subunit 1
- Interactome
- Gene Pathway
- Acute myeloid leukemia
- Adipocytokine signaling pathway
- Apoptosis
- B cell receptor signaling pathway
- Chemokine signaling pathway
- Chronic myeloid leukemia
- Epithelial cell signaling in Helicobacter pylori infection
- MAPK signaling pathway
- Metabolic pathways
- Neurotrophin signaling pathway
- Pancreatic cancer
- Pathways in cancer
- Prostate cancer
- Small cell lung cancer
- T cell receptor signaling pathway
- Toll-like receptor signaling pathway
- Related Disease
- Abortion, Spontaneous
- Acute Lung Injury
- Adenocarcinoma
- Alcoholism
- Alzheimer Disease
- Alzheimer disease
- Arthritis, Psoriatic
- Arthritis, Rheumatoid
- Asthma
- Atherosclerosis
- Atherosclerosis
- Behcet Syndrome
- Birth Weight
- Breast cancer
- Breast Neoplasms
- Calcinosis
- Carcinoid Tumor
- Carcinoma
- Carcinoma, Hepatocellular