L3MBTL polyclonal antibody
产品名称： L3MBTL polyclonal antibody
英文名称： L3MBTL polyclonal antibody
产品编号： PAB14105
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
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- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against L3MBTL.
- Immunogen:
- N-terminus of human L3MBTL.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Storage Buffer:
- In PBS (0.05% sodium azide, 0.05% proclin 300)
- Storage Instruction:
- Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- Western Blot (1:1000)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- The human L(3)MBT polycomb group protein is a transcriptional repressor and interacts physically and functionally with TEL (ETV6).
Boccuni P, MacGrogan D, Scandura JM, Nimer SD.J Biol Chem. 2003 Apr 25;278(17):15412-20. Epub 2003 Feb 14.
- Applications
- Western Blot (Cell lysate)
- Western blot was performed on HeLa nuclear extract (HeLa NE) using L3MBTL polyclonal antibody (Cat # PAB14105) at dilution 1 : 1,000 in TBS-Tween + 5% skimmed milk. The antibody recognizes endogenous L3MBTL1 in HeLa nuclear extract (HeLa NE). The location of L3MBTL1 is indicated with an arrow.
- Western Blot
- Western blot was performed using L3MBTL polyclonal antibody (Cat # PAB14105) at dilution 1 : 1,000.
The antibody recognizes the immunogen (GST-L1N) in the ng range (Lanes 1-3).
L3MBTL polyclonal antibody (Cat # PAB14105) also detects ectopically expressed FLAG-tagged L3MBTL1 (L3MBTL1-F) (Lane 5).
L3MBTL polyclonal antibody (Cat # PAB14105) also recognizes a protein of the correct size (approx. 100 kDa) (end. L3MBTL1 : endogenous L3MBTL1) in nuclear extract from HeLa (Lane 8) and HEK293 cells (Lane 7), but not in ML-2 cells (Lane 6).
A negative control (MOCK) is shown (Lane 4).
- Immunoprecipitation
- Immunoprecipitation was performed on nuclear extracts containing ectopically expressed FLAG-tagged L3MBTL1 (L3MBTL1-F) using L3MBTL polyclonal antibody (Cat # PAB14105) and an IgG control antibody (IgG Control).
The presence of L3MBTL1-F in the input (In ; Lane 1), in the precipitated fraction (P) and in the supernatant fraction (SN) was tested by Western Blot using anti-FLAG antibody.
L3MBTL1-F was efficiently precipitated by L3MBTL polyclonal antibody (Cat # PAB14105) (Lane 3), since there was no L3MBTL1-F present in the supernatant (Lane 5).
L3MBTL1-F was not precipitated by the control antibody (IgG Control) (Lane 2) and the L3MBTL1 was still present in the supernatant (Lane 4).
- Entrez GeneID:
- 26013
- Protein Accession#:
- Q9Y468
- Gene Name:
- L3MBTL
- Gene Alias:
- DKFZp586P1522,FLJ41181,H-L(3)MBT,KIAA0681,L3MBTL1,dJ138B7.3
- Gene Description:
- l(3)mbt-like (Drosophila)
- Omim ID:
- 608802
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene encodes the homolog of a protein identified in Drosophila as a suppressor of malignant transformation of neuroblasts and ganglion-mother cells in the optic centers of the brain. This gene product is localized to condensed chromosomes in mitotic cells. Overexpression of this gene in a glioma cell line results in improper nuclear segregation and cytokinesis producing multinucleated cells. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq
- Other Designations:
- OTTHUMP00000031024,l(3)mbt-like,lethal (3) malignant brain tumor l(3)