Phospho-DOK2 (Tyr351),磷酸化D酪氨酸激酶衰减蛋白2抗体

产品编号HZ-3101R
英文名称Phospho-DOK2 (Tyr351)
中文名称磷酸化D酪氨酸激酶衰减蛋白2抗体
别 名DOK2 (Phospho-Tyr351); DOK2 (Phospho Tyr351); DOK2 (Phospho Y351); Docking protein 2 56kDa; Docking protein 2 antibody DOK 2; DOK R; Downstream of tyrosine kinase 2; p56(dok 2); p56dok 2; p56DOK; p56dok2; DOK2; DOK2_HUMAN; Downstream of tyrosine kinase 2; OTTHUMP00000161710; OTTHUMP00000224923; p56(dok 2); p56(dok-2).
说 明 书0.1ml
产品类型磷酸化抗体
研究领域信号转导
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse,
Phospho-DOK2 (Tyr351),磷酸化D酪氨酸激酶衰减蛋白2抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量45kDa
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human p56Dok2 around the phosphorylation site of (Tyr351)
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Phospho-DOK2 (Tyr351),磷酸化D酪氨酸激酶衰减蛋白2抗体PubMedPubMed
产品介绍background:
The protein encoded by this gene is constitutively tyrosine phosphorylated in hematopoietic progenitors isolated from chronic myelogenous leukemia (CML) patients in the chronic phase. It may be a critical substrate for p210(bcr/abl), a chimeric protein whose presence is associated with CML. This encoded protein binds p120 (RasGAP) from CML cells. [provided by RefSeq, Jul 2008]

Function:
DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK2 may modulate the cellular proliferation induced by IL-4, as well as IL-2 and IL-3. May be involved in modulating Bcr-Abl signaling. Attenuates EGF-stimulated MAP kinase activation.

Subunit:
Interacts with phosphorylated RASGAP and EGFR. Interacts with RET and NCK. Interacts (via PH domain) with TEK/TIE2 (tyrosine phosphorylated).

Tissue Specificity:
Highly expressed in peripheral blood leukocytes, lymph nodes and spleen. Lower expression in thymus, bone marrow and fetal liver.

Post-translational modifications:
On immunoreceptor stimulation, phosphorylated on C-terminal tyrosine residues. Phosphorylation on Tyr-345 is required for binding to the SH2 domain of NCK. Phosphorylation on both Tyr-271 and Tyr-299 is required for interaction with RASGAP.

Similarity:
Belongs to the DOK family. Type A subfamily.
Contains 1 IRS-type PTB domain.
Contains 1 PH domain.

Gene ID:
9046

Phospho-DOK2 (Tyr351),磷酸化D酪氨酸激酶衰减蛋白2抗体Database links:
Entrez Gene: 9046 Human
Entrez Gene: 13449 Mouse
Omim: 604997 Human
SwissProt: O60496 Human
SwissProt: O70469 Mouse
Unigene: 71215 Human
Unigene: 243323 Mouse

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.