APOBEC1 polyclonal antibody
产品名称： APOBEC1 polyclonal antibody
英文名称： APOBEC1 polyclonal antibody
产品编号： PAB2473
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of APOBEC1.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human APOBEC1.
- Host:
- Rabbit
- Reactivity:
- Human, Mouse
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- The apolipoprotein B mRNA editing complex performs a multifunctional cycle and suppresses nonsense-mediated decay.
Chester A, Somasekaram A, Tzimina M, Jarmuz A, Gisbourne J, O'Keefe R, Scott J, Navaratnam N.EMBO J. 2003 Aug 1;22(15):3971-82.
- 2.
- A novel nuclear localization signal in the auxiliary domain of apobec-1 complementation factor regulates nucleocytoplasmic import and shuttling.
Blanc V, Kennedy S, Davidson NO.J Biol Chem. 2003 Oct 17;278(42):41198-204. Epub 2003 Aug 1.
- 3.
- Messenger RNA editing in mammals: new members of the APOBEC family seeking roles in the family business.
Wedekind JE, Dance GS, Sowden MP, Smith HC.Trends Genet. 2003 Apr;19(4):207-16.
- Applications
- Western Blot (Tissue lysate)
- The APOBEC1 polyclonal antibody (Cat # PAB2473) is used in Western blot to detect Apobec in mouse small intestine tissue lysate.
- Western Blot (Cell lysate)
- Western blot analysis of APOBEC1 polyclonal antibody (Cat # PAB2473) in 293 cell line lysates (35 ug/lane). APOBEC1 (arrow) was detected using the purified polyclonal antibody (1 : 60 dilution).
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with the APOBEC1 polyclonal antibody (Cat # PAB2473) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Entrez GeneID:
- 339
- Protein Accession#:
- NP_001635
- Gene Name:
- APOBEC1
- Gene Alias:
- APOBEC-1,BEDP,CDAR1,HEPR
- Gene Description:
- apolipoprotein B mRNA editing enzyme, catalytic polypeptide 1
- Omim ID:
- 600130
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene encodes a member of the cytidine deaminase enzyme family. The encoded protein forms a multiple-protein editing holoenzyme with APOBEC1 complementation factor (ACF) and APOBEC1 stimulating protein (ASP). This holoenzyme is involved in the editing of C-to-U nucleotide bases in apolipoprotein B and neurofibromatosis-1 mRNAs. [provided by RefSeq
- Other Designations:
- apolipoprotein B mRNA editing enzyme,apolipoprotein B mRNA editing enzyme complex-1
- Gene Pathway