Stat1 (phospho Y701) polyclonal antibody
产品名称： Stat1 (phospho Y701) polyclonal antibody
英文名称： Stat1 (phospho Y701) polyclonal antibody
产品编号： PAB11306
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of Stat1.
- Immunogen:
- Synthetic phosphopeptide corresponding to residues surrounding Y701 of mouse Stat1.
- Host:
- Rabbit
- Reactivity:
- Human, Mouse, Rat
- Specificity:
- This antibody is specific to mouse Stat1 protein phosphorylated at Y701.
- Form:
- Liquid
- Storage Buffer:
- In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Synthetic Peptide.
- Recommend Usage:
- ELISA (1:25000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- STAT1 regulates p73-mediated Bax gene expression.
Soond SM, Carroll C, Townsend PA, Sayan E, Melino G, Behrmann I, Knight RA, Latchman DS, Stephanou A.FEBS Lett. 2007 Mar 20;581(6):1217-26. Epub 2007 Mar 1.
- 2.
- Distinct modes of action applied by transcription factors STAT1 and IRF1 to initiate transcription of the IFN-gamma-inducible gbp2 gene.
Ramsauer K, Farlik M, Zupkovitz G, Seiser C, Kroger A, Hauser H, Decker T.Proc Natl Acad Sci U S A. 2007 Feb 20;104(8):2849-54. Epub 2007 Feb 9.
- 3.
- Murine norovirus 1 infection is associated with histopathological changes in immunocompetent hosts, but clinical disease is prevented by STAT1-dependent interferon responses.
Mumphrey SM, Changotra H, Moore TN, Heimann-Nichols ER, Wobus CE, Reilly MJ, Moghadamfalahi M, Shukla D, Karst SM.J Virol. 2007 Apr;81(7):3251-63. Epub 2007 Jan 17.
- Applications
- Western Blot (Cell lysate)
- Western blot using Stat1 (phospho Y701) polyclonal antibody (Cat # PAB11306) shows detection of phos-phorylated Stat1 (indicated by arrowhead at ~91 kDa) in K-562 cells after 30 min treatment with 1 ku of hIFN-alpha (lane 2).
No reactivity is seen for non-phosphorylated Stat1 in untreated cells (lane 1).
The primary antibody at a 1:1,000 dilution at 4°C, overnight.
Personal Communication from Ana Gamero, CCR-NCI, Bethesda, MD.
- Interactome
