CENPA,着丝粒蛋白A抗体

产品编号HZ-2753R
英文名称CENPA
中文名称着丝粒蛋白A
别 名CENP A; CENP-A; Centromere autoantigen A; Centromere protein A 17kDa; centromere protein A; centromeric protein A; Histone H3 like centromeric protein A; cenpa; CENPA_HUMAN; Histone H3-like centromeric protein A.
说 明 书0.1ml 0.2ml
研究领域细胞生物 染色质和核信号 细胞周期蛋白
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit,
CENPA,着丝粒蛋白A抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量15kDa
细胞定位细胞核
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human CENPA C-terminus
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
CENPA,着丝粒蛋白A抗体PubMedPubMed
产品介绍background:
Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. CENPA encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. CENPA is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq].

Function:
Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. May serve as an epigenetic mark that propagates centromere identity through replication and cell division. The CENPA-H4 heterotetramer can bind DNA by itself (in vitro).

Subunit:
Forms a nucleosome-like histone octamer containing two molecules each of H2A, H2B, CENPA and H4 assembled in one CENPA-H4 heterotetramer and two H2A-H2B heterodimers. Nucleosomes containing CENPA also contain histone H2A variants such as macroH2A H2AFY and H2A.Z/H2AFZ. The CENPA-H4 heterotetramer is more compact and structurally more rigid than corresponding H3-H4 heterotetramers. Heterotrimer composed of HJURP, CENPA and histone H4, where HJURP interacts with the dimer formed by CENPA and histone H4 and prevents tetramerization of CENPA and H4. Component of the CENPA-NAC complex, at least composed of CENPA, CENPC, CENPH, CENPM, CENPN, CENPT and MLF1IP/CENPU. Interacts (via CATD domain) with HJURP; the interaction is direct and is required for its localization to centromeres. Interacts with CENPC, CENPN and CENPT; interaction is direct. Interacts directly with herpes virus HSV-1 ICP0 protein. Part of a centromere complex consisting of CENPA, CENPT and CENPW.

CENPA,着丝粒蛋白A抗体Subcellular Location:
Nucleus. Chromosome, centromere, kinetochore. Note=Localizes exclusively in the kinetochore domain of centromeres. Occupies a compact domain at the inner kinetochore plate stretching across 2 thirds of the length of the constriction but encompassing only one third of the constriction width and height.

Tissue Specificity:
Ubiquitinated (Probable). Interaction with herpes virus HSV-1 ICP0 protein, leads to its degradation by the proteasome pathway.
Phosphorylation of Ser-7 by AURKA and AURKB during prophase is required for localization of AURKA and AURKB at inner centromere and is essential for kinetochore function. Initial phosphorylation during prophase is mediated by AURKA and is maintained by AURKB.
Poly-ADP-ribosylated by PARP1.

Similarity:
Belongs to the histone H3 family.

Gene ID:
1058

Database links:
Entrez Gene: 1058 Human
Omim: 117139 Human
SwissProt: P49450 Human
Unigene: 1594 Human

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

CENP-A可将着丝粒变成一个DNA和蛋白的复合物，而且确保着丝粒在细胞分裂中完好无损。
CENP-A的存在确保了人体有几乎完全相同的染色体组。
CENP-A功能失常将导致细胞分裂过程中发生染色体分离异常。
CENP-A对于染色体着丝粒的位置起决定作用，因而被认为是重要的表观遗传学标记蛋白。
CENP-A改变了核小体的性状，使得它变得更加坚硬。核小体是由DNA与组合蛋白八聚体构成的真核染色体的一种重复结构，核小体的组织对于基因调控极为重要。不同于染色质上的其他区域。
CENP-A取代组蛋白H3形成核小体，CENP-A核小体重复拷贝形成一个特异的表观遗传区域。